Edible mushroom polysaccharides can dissolve in water, acid, alkali, salt solutions, but are insoluble in organic solvents such as alcohols, ethers, and ketones. Therefore, the extraction of polysaccharides often uses methods such as water extraction and alcohol precipitation, acid-base extraction, enzymatic extraction, ultrasonic extraction, microwave extraction, and supercritical fluid extraction. At present, there have been many studies on the extraction process of polysaccharides from Flammulina velutipes. The basic process is as follows: fresh Flammulina velutipes or dried Flammulina velutipes → crushing → hot water extraction (distilled water or dilute alkali solution or dilute acid solution) or enzymatic extraction (single enzyme method or compound enzyme method) or ultrasonic extraction → concentration → ethanol precipitation → protein removal → washing → drying → crude polysaccharides from Flammulina velutipes. The main methods for protein removal include Savage method, trifluorotrichloroethane method, trichloroacetic acid method, etc. Among them, Savage method is the most commonly used, which uses chloroform and n-butanol to treat polysaccharide aqueous solution, denature and precipitate protein components, and centrifuge for separation. In the above process, there are many factors that affect the extraction rate of polysaccharides from Flammulina velutipes, including temperature, solid-liquid ratio, extraction time, extraction frequency, and extraction method.